Mammalian expression
We have recently started a mammalian transient expression pipeline in late 2017, by using ExPi cell lines (ThermoFisher) and a selection of expression plasmids. Our priority is given to secreted proteins, cell-surface antigens, antibodies, membrane proteins and multi-subunit complexes. Please contact us for more details.
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In-silico to in-vitro
Use the LIMS to create and manage your protein constructs
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High-throughput cloning
The platform will clone your protein constructs into E. coli
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High-throughput protein purification
Your protein constructs will be purified using IMAC and SEC
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Purification using affinity chromatography
All constructs will be fused to a N-terminal cleavable HIS6-tag
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Expression in insect cells for difficult proteins
We are currently implementing high-throughput cloning using baculovirus
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Detergent screening
Transmembrane proteins will be screened for optimal detergent conditions
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Multiple quality control steps
All protein batches will be verified using mass spectrometry and/or sequencing
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We deliver!
Receive plasmids, expression strain glycerol stocks and protein batches
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