The Protein Production Platform
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 Contact us at mwchen@ntu.edu.sg for more information.


About   Working with PPP   Cloning   Large-scale expression   BEVS   Mammalian expression   Expression vectors   FAQ   



The Protein Production Platform of NTU

Welcome to the Protein Production Platform (PPP) at NTU School of Biological Sciences. We have created this website to make our collaboration with you as smooth as possible. You will find a detailed description of our pipeline as well as instructions for submitting your targets and constructs on this page.

We use technology and a workflow that is largely identical to that of the former Biotechnology unit at the Structural Genomics Consortium at Karolinska Institutet in Stockholm, Sweden. In addition, we create in-house solutions such as new expression vectors to better serve your protein production needs.


  • In-silico to in-vitro
    Use the LIMS to create and manage your protein constructs
  • High-throughput cloning
    The platform will clone your protein constructs into E. coli
  • High-throughput protein purification
    Your protein constructs will be purified using IMAC and SEC
  • Purification using affinity chromatography
    All constructs will be fused to a N-terminal cleavable HIS6-tag
  • Expression in insect cells for difficult proteins
    We are currently implementing high-throughput cloning using baculovirus
  • Detergent screening
    Transmembrane proteins will be screened for optimal detergent conditions
  • Multiple quality control steps
    All protein batches will be verified using mass spectrometry and/or sequencing
  • We deliver!
    Receive plasmids, expression strain glycerol stocks and protein batches

In short, we are a high-throughput protein production facility with a cloning capacity of roughly 2*96 clones per month and a large-scale purification capacity of 12 proteins per week by using Escherichia coli as expression host. We also perform cloning, small-scale expression tests and large-scale protein expression in insect cells and most recently, mammalian cells. Please contact us for more details.